莫维,郝俊光,侯慧,祁岑,林海潮,陈宏红,罗东伟

• 1:广西大学轻工与食品工程学院
• 2:北部湾大学钦州市食品风味分析调控重点实验室
• 3:北部湾大学广西高校北部湾海产品高值化利用与预制食品重点实验室
• 4:广西大益生态食品有限公司

摘要(Abstract)：

为向毛葡萄和桑椹深加工产品提供品控支持，开发了同时测定毛葡萄汁和桑椹汁及毛葡萄醋和桑椹醋中18种酚类物质的高效液相色谱方法，并建立了酚类物质的外标法定量。使用Atlantis~?T3色谱柱(4.6 mm×250 mm×5μm),以含1%乙酸的甲醇-乙腈(4∶6)为流动相A及1%乙酸-水溶液为流动相B,柱温30℃,流速1 mL/min,进样量5μL。通过洗脱梯度优化，实现了待测酚类物质的有效分离。方法学验证表明，18种酚类物质的标准曲线线性关系均≥0.993 6,平均加标回收率在88.00%～107.46%之间，对应的RSD≤4.38%。结果表明，该方法操作简单，准确度高，可应用于工厂的毛葡萄汁和桑椹汁及毛葡萄醋和桑椹醋中酚类物质的定量品控。

关键词(KeyWords)： 高效液相;桑椹;毛葡萄;酚类物质;果汁;果醋

Abstract:

Keywords:

基金项目(Foundation): 广西壮族自治区大学生创新创业训练项目(202211607015);; 广西壮族自治区科技计划项目(桂科AB21238006);; 北部湾大学高层次人才启动项目(2018KYQD53)

作者(Author): 莫维,郝俊光,侯慧,祁岑,林海潮,陈宏红,罗东伟

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