利用HPLC和GC全面分析葡萄醋中有机酸Determination of carboxylic acids in grape vinegars by HPLC and GC methods
张颖,张健
摘要(Abstract)：
葡萄醋成分复杂,对有机酸的准确测定影响很大,本实验采用两步固相萃取技术除杂,比较了外标法和标准加入法定量的优劣,分析了HPLC和GC方法各自的局限性。
关键词(KeyWords)： 葡萄醋;有机酸;固相萃取;高效液相色谱法;气相色谱法
基金项目(Foundation):
作者(Author): 张颖,张健
参考文献(References)：
- [1]陈义伦,裘立群,陈伟,等.原汁苹果醋中的有机酸[J].食品与发酵工业,2004,30(8):92-95.
- [2]Garcia Romero,E.,Sanchez Mun?oz,G.Determination of organicacids,in grapemusts,wines and vinegars by high perfor-manceliquid chromatography[J].J.Chroma-togr.A 1993,655,111-117.
- [3]Zerbinati,O,Aigotti,R.,Daniele,P.G.A-nalysis of chiralcarboxylic acids in wine by highperformance liquid chromatographywith cou-pled UV and circular dichroism detection[J].J.Chromatogr.A 1994,671,281-285.
- [4]Calull,M.;Marce`,R.M.;Borrull,F.De-termination of carboxylicacids,sugars,glycer-ol and ethanol in wine and grape must byionexchange high performance liquid chromatogra-phy withrefractive index detection[J].J.Ch-romatogr.1992,590,215-222.
- [5]Sanarico,D;Bertolini L;Antonelli A.HPLCDetermination of Organic Acids in TraditionalBalsamic Vinegar of Reggio Emilia[J].LiquidChromatography&RelatedTechnologies,2003,26(13),2177-2187.
- [6]Angelo G.Giumanini;Giancarlo Verardo;Daniele Della Martina;Nicoletta Toniutti.Improved Method for the Analysis of OrganicAcids and New Derivatization of Alcohols inComplex Natural Aqueous Matrixes:Applica-tion to Wine and Apple Vinegar[J].J.Agric.Food Chem.,2001,49,2875-2882.
- [7]Community Bureau of Reference.BCR Refer-ence Materials;Commission of the EuropeanCommunities:Brussells,Belgium.
- [8]Funk,W.,Dammann,V.,Donnevert,G.,Eds.Establishing a new analytical procedure.InQualityAssuranceinAnalyticalChemistry;VCH:Weinheim,Germany,1995;pp 15-57.
- [9]汪正范.色谱定性与定量[M].北京:化学工业出版社,2000,169-175.
- [10]Mentasti,E.,Gennaro,M.C.,Sarzanini,C.,Baiocchi,C.et al.Derivatisation,identification and separation of carboxylicacids in wines and beverages by high perform-ance liquid chromatography[J].J.Chrom-atogr.1985,322,177-189.
- [11]Vonach,R.;Lendl,B.;Kellner,R.Highperformance liquid chromatography with realtime Fourier transform infrared detection fordetermination of carbohydrates,alcohols an-dorganic acids in wines[J].J.Chromatogr.A 1998,824,159-167.