刘玲玲,王强,高晶,张正方,唐军

• 1:新疆大学理化测试中心

摘要(Abstract)：

采用2,2'连氨-双-(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS)和二苯代苦味酰基自由基(DPPH),还原能力法对阿育魏实抗氧化活性进行研究,并测定了各样品中总酚和总黄酮含量。结果表明:阿育魏实挥发油和甲醇提取物具有明显的抗氧化能力,清除DPPH.IC50为0.22mg/mL和0.16mg/mL,ABTS.IC50为3.82mg/mL和3.20mg/mL。在3mg/mL时,挥发油和不同溶剂提取物中甲醇提取物表现出较好的还原能力。挥发油和提取物抗氧化活性与酚类和黄酮类化合物具有很大相关性。

关键词(KeyWords)： 阿育魏实;溶剂萃取;抗氧化;总酚总黄酮

Abstract:

Keywords:

基金项目(Foundation): 新疆维吾尔自治区高校科研计划项目(XJUEDU2011I04)

作者(Author): 刘玲玲,王强,高晶,张正方,唐军

参考文献(References)：

• [1]阿布都热依木.卡地尔.中华本草——维吾尔药卷[M].上海:上海科学技术出版社,2005:200-201.
• [2]中华人民共和国药典委员会.中华人民共和国卫生部药品标准维吾尔药分册[M].乌鲁木齐:新疆科技卫生出版社,1999:42.
• [3]Zek Gulmira,Fatih Demirci,Temel zek,et al.Gas chroma-tographic-mass spectrometric analysis of volatiles obtainedby four different techniques from Salvia rosifolia Sm.,andevaluation for biological activity[J].Journal of Chromatog-raphy A,2010,1217(5):741-748.
• [4]刘力,张惠,宋铁珊,等.阿育魏实挥发油成分的GC-MS分析[J].分析测试学报,2004,23(3).
• [5]Khan R M,Zakir Z,Khanam,et al.Novel compound fromTrachyspermum ammi(Ajowan caraway)seeds with anti-biofilm and antiadherence activities against Streptococcusmutans:apotential chemotherapeutic agent against dentalcaries[J].Journal of Applied Microbiology,2010,109(6):2151-2159.
• [6]Pandey Sk,S Upadhyay,Ak Tripathi.Insecticidal and repellentactivities of thymol from the essential oil of Trachyspermum ammi(Linn)Sprague seeds against Anopheles stephensi[J].Parasitol-ogy research,2009,105(2):507-512.
• [7]吴霞.维吾尔药阿里红、阿育魏实的化学成分及生物活性研究[D].北京:中国协和医科大学,2005.
• [8]Conforti F,S Sosa,M Marrelli,et al.The protective abilityof Mediterranean dietary plants against the oxidative dam-age:The role of radical oxygen species in inflammation andthe polyphenol,flavonoid and sterol contents[J].Foodchemistry,2009,112(3):587-594.
• [9]李品艾,睢超霞,张玲.花椒黄酮类化合物提取液清除自由基作用研究[J].中国调味品,2012,37(8):23-25.
• [10]Singleton Vernon L,Rudolf Orthofer,Rosa M,et al.A-nalysis of total phenols and other oxidation substrates andantioxidants by means of folin-ciocalteu reagent,in Meth-ods in Enzymology,P.Lester,Editor.Academic Press.1999:152-178.
• [11]国家药典委员会.中国药典一部附录[M].北京:化学工业出版社,2010:36.
• [12]Krishnaiah Duduku,Rosalam Sarbatly,Rajesh Nithyanan-dam.A review of the antioxidant potential of medicinalplant species[J].Food and Bioproducts Processing,2011,89(3):217-233.
• [13]李楠,祖元刚,王微.芹菜籽精油的抑菌与抗氧化作用研究[J].中国调味品,2012,37(5):28-31.
• [14]王强,刘玲玲,唐军,等.新疆产孜然抗氧化活性研究[J].中国实验方剂学杂志,2012(16):42.
• [15]王强,殷超,彭秧.比较研究新疆小茴香不同溶剂提取物的抗氧化活性[J].中国酿造,2012,31(6):95-97.
• [16]Sahreen Sumaira,Muhammad Rashid Khan,Rahmat AliKhan.Evaluation of antioxidant activities of various sol-vent extracts of Carissa opaca fruits[J].Food chemistry,2010,122(4):1205-1211.