李玲,陈凯,王贻莲,张薇,杨玉忠,扈进冬,李纪顺

• 1:山东省科学院生态研究所山东省应用微生物重点实验室
• 2:农业部农药检定所

摘要(Abstract)：

为了提高Monacolin K的产量,应用30keV的N+离子注入,对紫红曲霉M2进行诱变育种。诱变剂量分别为1×10~(15),5×10~(15),1×1016,5×1016,1×1017 N+/cm2,通过构巢曲霉对峙培养、洛伐他汀抗性筛选、高效液相色谱HPLC方法筛选高产Monacolin K的优秀突变菌株,并且通过液态发酵试验优化部分培养条件。结果显示:相对原菌株M2,最佳诱变菌株11-13产Monacolin K的能力是原菌株M2的2.3倍,连续传代5次,其高产遗传形状稳定。其最佳发酵培养条件为:温度30℃,接种量10%,转速200r/min,发酵时间14天。

关键词(KeyWords)： 红曲霉;Monacolin K;N+离子注入;诱变育种;发酵条件

Abstract:

Keywords:

基金项目(Foundation): 山东省科学院青年基金项目(2017QN009)资助;; 山东省重点研发计划(2016GNC113009)

作者(Author): 李玲,陈凯,王贻莲,张薇,杨玉忠,扈进冬,李纪顺

参考文献(References)：

• [1]Cheng M J,Wu M D,Chan H Y,et al.A new azaphilone metabolite from the fungus Monascus ruber[J].Chem Nat Compd,2016,52:231-233.
• [2]Cheng M J,Wu M D,Chen J J,et al.Secondary metabolites from the red mould rice of Monascus pupureus BCRC38113[J].Nat Prod Res,2010,24:1719-1725.
• [3]Sham T T,Chan C O,Wang Y H,et al.A review on the traditional Chinese medicinal herbs and formulae with hypolipidemic effect[J].Biomed Res Int,2014:21-24.
• [4]Liu M T,Wang A L,Zhen S,et al.Cytotoxic monacolin analogs from Monascus pupureus fermented rice[J].J Asian Nat Prod Res,2013,15:600-609.
• [5]Dajung J,Deokyeong C,Kyunghwa N,et al.Biological evaluation of novel derivatives of the orange pigments fromMonascus pupureus sp.as inhibitors of melanogenesis[J].Biotechnol Lett,2014,36:1605-1613.
• [6]Su Y C,Wang J J,Lin T T,et al.Production of the secondary metabolitesγ-aminobutyric acid and monacolin K by Monascus[J].J Ind Microbiol Biotechnol,2003,30:41-46.
• [7]Endo A.Monacolin K,a new hypocholesterolemic agent produce by aMonascus species[J].J Antibiot,1979,32(8):852-854.
• [8]Alberts A W,Chen J,Kuron G,et al.Mevinolin,a highly protent competitive inhibitor of hydroxymethylglutaryl coenzyme A reduciase and a cholesterol-lowering agent[J].P Natl Acad Sci,1980,77(7):3957-3961.
• [9]Endo A.Monacolin K,a new hypocholesterolemic agent that specifically inhibits 3-hydroxy-3-methylglutaryl coenzyme a reductase[J].J Antibiot,1980,33(3):334-336.
• [10]Feuerstein J,Bjerke W.P02.88.powdered red yeast rice and plant stanols and sterols to lower cholesterol[J].BMC Complem Altern,2012,9(2):110-115.
• [11]Chen H H,Chen Y Y,Yeh J Z,et al.Immune-stimulated antitumor effect of different molecular weight polysaccharides from Monascus pupureus on human leukemic U937cells[J].Cyta-J Food,2014,12:134-140.
• [12]Patel S.Functional food red yeast rice(RYR)for metabolic syndrome amelioration:a review on pros and cons[J].World J Microb Biot,2016,32(87):1-12.
• [13]Klawitter J,Shokati T,Moll V,et al.Effects of lovastatin on breast cancer cells:aproteo-metabonomic study[J].Breast Cancer Res,2010,12(2):1-20.
• [14]Francielo V,Rose M B,Julio C C,et al.Monascus:a reality on the production and application of microbial pigments[J].Appl Biochem Biotech,2016,178:211-223.
• [15]缪建顺,杨建设,张苗苗,等.重离子辐照微生物效应及诱变育种进展[J].辐射研究与辐射工艺学报,2014,32(2):1-8.
• [16]朱华,许赣荣,陈蕴.HPLC法测定红曲中酸型与内酯型Monacolin K[J].无锡轻工大学学报,2003,22(3):46-52.
• [17]邵春林,余增亮.离子束辐照下微生物、植物组织存活模型的研究[J].核技术,1997,20(7):423-430.
• [18]宋道军,姚建铭,邵春林,等.离子注入微生物产生“马鞍型”存活曲线的可能作用机制[J].核技术,1999,22(3):129-132.
• [19]Joiner M C,Marples B,Lambin P,et al.Low-dose hypersensitivity:current status and possible mechanisms[J].Int J Radiat Oncol Biol Phys,2001,49(2):379-389.
• [20]韩荣飞,吴跃进,卞坡,等.低能重离子剂量-存活率效应及其拟合模型探讨[J].原子核物理评论,2009,26(4):352-355.
• [21]陈泉,吴远征,扈进冬,等.高产Monacolin K红曲霉菌种的筛选及液态发酵条件优化[J].中国酿造,2015,34(6):43-47.