吕明帅,赵博,孙文玉,万水晶,刘春延,张国财

• 1:东北林业大学林学院

摘要(Abstract)：

采用超声辅助法对富硒黑木耳多糖和普通黑木耳多糖进行提取和分离纯化,得到富硒黑木耳多糖(Se-AAP-2)和普通黑木耳多糖(AAP-2),比较分析Se-AAP-2和AAP-2的分子量、单糖组成、纯度、糖醛酸含量、体外及体内抗氧化活性。结果表明,Se-AAP-2和AAP-2均由甘露糖和葡萄糖组成,分子摩尔比分别为1∶1.31和1∶1.28;相比AAP-2,Se-AAP-2的平均分子量降低了23.5%(P<0.01),糖醛酸含量提高了33.7%(P<0.01),说明富硒能够显著降低黑木耳多糖的分子量,提升其糖醛酸含量。此外,Se-AAP-2拥有更高的体外抗氧化活性,相比AAP-2,其总还原力提高了11.4%,羟基自由基和ABTS自由基清除能力IC_(50)亦分别降低了42.2%和26.4%。体内抗氧化活性评价结果表明,Se-AAP-2相较于AAP-2具有更强的体内抗氧化活性,能够显著提高小鼠肝、肾、心、血清中T-SOD、GSH-Px的活性,降低其MDA含量。

关键词(KeyWords)： 黑木耳;硒;多糖;抗氧化活性

Abstract:

Keywords:

基金项目(Foundation): 中央财政林业科技推广示范资金项目([2019]HZT02);; 哈尔滨市应用技术研究与开发项目(2016RAXXJ035)

作者(Author): 吕明帅,赵博,孙文玉,万水晶,刘春延,张国财

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