崔贻媛,高伦江,郭政航,戴凤仪,游玉明

• 1:重庆文理学院智慧农业学院
• 2:重庆市农业科学院农产品加工研究所

摘要(Abstract)：

为探讨辣椒素的高效、绿色提取方法，该研究采用低共熔溶剂协同超声辅助提取技术，以辣椒素提取量为考察指标，在筛选低共熔溶剂组合的基础上，通过单因素试验和响应面法对低共熔溶剂含水量、提取时间、提取温度、液料比等关键参数进行优化。结果表明，以摩尔比为1∶1的甜菜碱和DL-苹果酸为提取溶剂，在提取时间为52 min、提取温度为46℃、液料比为45∶1、低共熔溶剂含水量为32%的条件下，辣椒素提取量达到4.01 mg/g,较传统乙醇法提升47.43%。该方法绿色、环保，可为辣椒素的高效提取提供科学参考。

关键词(KeyWords)： 辣椒素;低共熔溶剂;超声辅助;响应面法

Abstract:

Keywords:

基金项目(Foundation): 国家级大学生创新创业训练计划项目(202510642010);; 重庆市技术创新与应用发展专项(CSTB2022TIAD-KPX0085)

作者(Author): 崔贻媛,高伦江,郭政航,戴凤仪,游玉明

参考文献(References)：

• [1]Abulaiti A,Naseer Z,Ahmed Z,et al.Dietary supplementation of capsaicin enhances productive and reproductive efficiency of Chinese crossbred buffaloes in low breeding season[J].Animals,2023,13(1):118.
• [2]Li Jun,Liao Xiaojun,Yin Xuedong,et al.Gut microbiome and serum metabolome profiles of capsaicin with cognitive benefits in APP/PS1 mice[J].Nutrients,2023,15(1):118.
• [3]Behbehani J M,Irshad M,Shreaz S,et al.Anticandidal activity of capsaicin and its effect on ergosterol biosynthesis and membrane integrity of Candida albicans[J].International Journal of Molecular Sciences,2023,24(2):1046.
• [4]中国产业研究院.2024—2029年调味品产业现状及未来发展趋势分析报告[R].2024-05.
• [5]崔桂娟，谢秋涛，张群，等.辣椒中辣椒素提取方法与应用[J].食品工业科技，2018,39(13):306-310.
• [6]程立坤，胡悦，张亚琨，等.不同品种辣椒营养品质、辣椒红素含量的差异比较及聚类分析[J].中国调味品，2024,49(1):133-140.
• [7]De Aguiar A C,Osorio-Tobón J F,Silva L P S,et al.Economic analysis of oleoresin production from malagueta peppers (Capsicum frutescens) by supercritical fluid extraction[J].The Journal of Supercritical Fluids,2018,133:86-93.
• [8]Jiang Zhengmeng,Wang Lanjin,Gao Zhao,et al.Green and efficient extraction of different types of bioactive alkaloids using deep eutectic solvents[J].Microchemical Journal,2019,145:345-353.
• [9]仝洺慧，朱越，李佳凝，等.低共熔溶剂对黄连中生物碱绿色提取工艺的优化[J].中成药，2023,45(9):3014-3017.
• [10]邱菊，陈昌琳，陈冰艺，等.响应面法优化超声辅助乙醇提取桑葚花青素工艺及稳定性分析[J].中国调味品，2023,48(11):176-182.
• [11]Milo??evi■,et al.Use of natural deep eutectic solvent (NADES) as a green extraction of antioxidant polyphenols from strawberry tree fruit (Arbutus unedo L.):an optimization study[J].Microchemical Journal,2024,200:110284.
• [12]Wojeicchowski J P,Ferreira A M,Abranches D O,et al.Using COSMO-RS in the design of deep eutectic solvents for the extraction of antioxidants from rosemary[J].ACS Sustainable Chemistry & Engineering,2020,8:12132-12141.
• [13]Khokhar V,Dhingra D,Pandey S.Effect of temperature and composition on density and dynamic viscosity of (lanthanide metal salts+urea) deep eutectic solvents[J].Journal of Molecular Liquids,2022,360:119396.
• [14]Lin Hanqing,Gong Ke,Hykys P,et al.Nanoconfined deep eutectic solvent in laminated MXene for efficient CO2 separation[J].Chemical Engineering Journal,2021,405:126961.
• [15]Sirvi?? J A,Haataja R,Kantola A M,et al.Insights into the role of molar ratio and added water in the properties of choline chloride and urea-based eutectic mixtures and their cellulose swelling capacity[J].Physical Chemistry Chemical Physics,2022,46:28609-28620.
• [16]Cea-Klapp E,Gajardo-Parra N,Aravena P,et al.Interfacial properties of deep eutectic solvents by density gradient theory[J].Industrial & Engineering Chemistry Research,2022,61(6):2580-2591.
• [17]Wang Jiaqin,Jing Wenqiang,Tian Haiyuan,et al.Investigation of deep eutectic solvent-based microwave-assisted extraction and efficient recovery of natural products[J].ACS Sustainable Chemistry & Engineering,2020,8(32):12080-12088.
• [18]刘文杰，白春华，刘庆明，等.高挥发性液体传质速率机理和实验研究[J].兵工学报，2020,41(6):1123-1130.
• [19]Sultana B,Anwar F,Ashraf M.Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts[J].Molecules,2009,14(6):2167-2180.
• [20]Wu Zhiyuan,Zuo Lijie,Zhang Hongliang,et al.Effect of liquid-solid volume ratio and surface treatment on microstructure and properties of Cu/Al bimetallic composite[J].Crystals,2023,13(5):794.
• [21]Chatzimitakos T,Athanasiadis V,Kotsou K,et al.Optimization of pressurized liquid extraction (PLE) parameters for extraction of bioactive compounds from Moringa oleifera leaves and bioactivity assessment[J].International Journal of Molecular Sciences,2024,25(9):4628.
• [22]Wang Yan,Cai Wensheng,Shao Xueguang.Near-infrared spectroscopy for the analysis of water structure in low eutectic solvents[J].Journal of Higher Education Chemistry,2023,44(6):39-44.
• [23]刘佳怡，黄磊磊，王天怡，等.超声协同低共熔溶剂提取紫丁香花多酚工艺优化及抗氧化活性分析[J].食品工业科技，2024,45(4):171-179.
• [24]Nava-Ocampo M F,Al Fuhaid L,Santana A,et al.Structural properties and stability of the Betaine-Urea natural deep eutectic solvent[J].Journal of Molecular Liquids,2021,343:117655.