基于多元统计分析枸杞原浆加工过程中品质动态变化及综合评价的研究Study on Dynamic Changes in Quality and Comprehensive Evaluation of Wolfberry Pulp During Processing Based on Multivariate Statistical Analysis
梁丽鲜,孟新涛,曹雪琴,刘一鸣,许铭强,马燕,张婷,潘俨
摘要(Abstract):
为探究枸杞原浆加工过程中品质动态变化特性和关键影响工段,该研究以新疆枸杞为研究对象,结合气相色谱-离子迁移谱(gas chromatography-ion mobility spectroscopy, GC-IMS)技术和理化、营养指标分析方法,测定不同加工工段(榨汁、钝酶、胶体磨、高压均质、超声脱气、灭菌)品质动态变化规律,并采用正交偏最小二乘判别分析(orthogonal partial least squares discriminant analysis, OPLS-DA)和聚类分析(clustering analysis, CA)进行综合评价。结果表明,与榨汁工段相比,钝酶后枸杞原浆中总酚、多糖、甜菜碱的含量分别降低了28.17%、12.49%、32.32%,经胶体磨至超声加工工段后,理化、营养指标较钝酶工段整体维持稳定且呈平缓上升趋势,甜菜碱含量和总酚含量在胶体磨工段分别提高至8.91 mg/g和8.77 mg/g, V_C含量和多糖含量在高压均质工段分别提高至235.87 mg/100 g和34.22 mg/g,超声脱气处理后总酚含量和类胡萝卜素含量分别提高至8.72 mg/g和2.40 mg/g,灭菌加工后,类胡萝卜素含量损失62.08%,总矿物质含量损失17%,呈味氨基酸含量损失50%以上。采用GC-IMS共鉴定出53种挥发性化合物,包括醇类15种、酯类7种、醛类16种、酮类5种、其他类10种,OPLS-DA共筛选出20种关键挥发性化合物,其中醇类为榨汁工段主要挥发性化合物,醛类为灭菌工段主要挥发性化合物。综上所述,钝酶和灭菌工段是影响枸杞原浆品质的关键加工工段,因此,研究其加工过程中品质变化规律可为枸杞产业的标准化生产和关键工段的精准调控提供理论依据。
关键词(KeyWords): 枸杞原浆;加工过程;品质变化;多元分析;综合评价
基金项目(Foundation): 新疆维吾尔自治区重点研发项目课题(2023B02009-1);; 新疆维吾尔自治区公益性科研院所基本科研业务费专项(KY2022013)
作者(Author): 梁丽鲜,孟新涛,曹雪琴,刘一鸣,许铭强,马燕,张婷,潘俨
参考文献(References):
- [1]Amagase H,Farnsworth N R.A review of botanical characteristics,phytochemistry,clinical relevance in efficacy and safety of Lycium barbarum fruit (Goji)[J].Food Research International,2011,44(7):1702-1717.
- [2]邢丽杰,罗力力,向晓黎,等.新疆枸杞中功能性营养成分分析研究[J].农产品质量与安全,2021(4):43-46.
- [3]Liu Yuxuan,Fang Haitian,Liu Huiyan,et al.Goji berry juice fermented by probiotics attenuates dextran sodium sulfate-induced ulcerative colitis in mice[J].Journal of Functional Foods,2021,83:104491.
- [4]Yu Jing,Yan Yamen,Zhang Lutao,et al.A comprehensive review of goji berry processing and utilization[J].Food Science & Nutrition,2023,11(12):7445-7457.
- [5]苏生元,张晓燕,孟芳,等.不同商业酿酒酵母发酵的枸杞酒品质分析[J].中国酿造,2024,43(12):196-204.
- [6]韩国玮,胡晓霞,杨戈斌.枸杞与黄芪酿造醋营养成分的对比分析[J].中国调味品,2023,48(4):183-186.
- [7]刘海龙,张卿,王家枢,等.乳酸菌发酵型枸杞果酱的研制及贮藏品质分析[J].中国调味品,2025,50(9):102-109.
- [8]Xia Ting,Qiang Xiao,Geng Beibei,et al.Changes in the phytochemical and bioactive compounds and the antioxidant properties of wolfberry during vinegar fermentation processes[J].International Journal of Molecular Sciences,2022,23(24):15839.
- [9]Daniel H F,Tadamasa K,Tsuyoshi K,et al.Enzymatic browning,polyphenol oxidase activity,and polyphenols in four apple cultivars:dynamics during fruit development[J].Hortscience,2010,45(8):1150-1154.
- [10]Illera A E,Sagrario B,María T S.Enzyme inactivation and changes in the properties of cloudy apple juice after high-pressure carbon dioxide and thermosonication treatments and during refrigerated storage[J].Journal of Food Processing and Preservation,2020,44(7):14521.
- [11]Singh S V,Singh R,Verma K,et al.Effect of microfluidization on quality characteristics of sapodilla (Manilkara achras L.) juice[J].Food Research International,2022,162:112089.
- [12]Tian You,Sun Lijun,Yang Yali,et al.Changes in the physicochemical properties,aromas and polyphenols of not from concentrate (NFC) apple juice during production[J].CyTA-Journal of Food,2018,16(1):755-764.
- [13]Panozzo A,Lemmens L,Van Loey A,et al.Microstructure and bioaccessibility of different carotenoid species as affected by high pressure homogenisation:a case study on differently coloured tomatoes[J].Food Chemistry,2013,141(4):4094-4100.
- [14]徐艺洁.枸杞原浆加工过程中色泽变化与抑制方法的研究[D].武汉:华中农业大学,2023.
- [15]叶朵朵,孟新涛,古丽米热·祖努纳,等.非浓缩还原(NFC)枸杞汁加工过程中品质变化特性[J].新疆农业科学,2025,62(2):361-372.
- [16]王雨洁.黑枸杞沙棘复合果汁的工艺及贮藏品质的研究[D].银川:宁夏大学,2022.
- [17]叶朵朵.营养复合型NFC枸杞汁工艺优化及产品研发[D].乌鲁木齐:新疆农业大学,2024.
- [18]G??k??en B,Sevcan ??.Cold pressed vs.centrifugal juice:comparison in terms of the juice yield,physicochemical and phytochemical properties[J].Food Science and Engineering,2024,5:145.
- [19]Aime K N,Steve Z D C,Sirshendu D,et al.Pectinase hydrolysis of guava pulp:effect on the physicochemical characteristics of its juice[J].Heliyon,2021,7(10):8141.
- [20]Zubaidi M A,Czaplicka M,Kolniak-Ostek J,et al.Effect of different enzyme treatments on juice yield,physicochemical properties,and bioactive compound of several hybrid grape varieties[J].Molecules (Basel,Switzerland),2025,30(3):556.
- [21]Alshymaa K A.Antioxidant and mineral content of beverages based on permeate with fruit juice and green tea extract[J].Journal of Food and Dairy Sciences,2021,12(12):319-323.
- [22]Kim A N,Lee K Y,Rahman M S,et al.Thermal treatment of apple puree under oxygen-free condition:effect on phenolic compounds,ascorbic acid,antioxidant activities,color,and enzyme activities[J].Food Bioscience,2021,39:100802.
- [23]Pedrosa F D L,Kouzounis D,Schols H,et al.Assessing high-temperature and pressure extraction of bioactive water-soluble polysaccharides from passion fruit mesocarp[J].Carbohydrate Polymers,2024,335:122010.
- [24]Ding Xuefeng,Zhang Yanbei,Li Jie,et al.Structure spectral properties and antioxidant activity of melanoidins extracted from high temperature sterilized lotus rhizome juice[J].International Journal of Biological Macromolecules,2024,270:132171.
- [25]纵伟,张丽华,张沙沙,等.胶体磨处理对鲜枣浆黏度特性的影响[J].郑州轻工业学院学报(自然科学版),2014,29(6):25-27.
- [26]Suo Guanwen,Zhou Chunli,Su Wei,et al.Effects of ultrasonic treatment on color,carotenoid content,enzyme activity,rheological properties,and microstructure of pumpkin juice during storage[J].Ultrasonics Sonochemistry,2022,84:105974.
- [27]Lan Tian,Wang Jiaqi,Bao Shihan,et al.Effects and impacts of technical processing units on the nutrients and functional components of fruit and vegetable juice[J].Food Research International,2023,168:112784.
- [28]Zhang Zhiwei,Zhang Meiyue,Gao Zhenhong,et al.Effect of dynamic high-pressure microfluidization on the quality of not-from-concentrate cucumber juice[J].Foods (Basel,Switzerland),2024,13(13):2125.
- [29]Espaňa C M A,Ramírez G A M,Buenfil R M I,et al.Synthesis mechanism and therapeutic effects of thiosulfinates and polysulfides of different species of garlic from the Allium genus[J].Pharmaceutics,2025,17(4):437.
- [30]Cerrillo I,Fernández-Pachón M S,Collado-González J,et al.Effect of fermentation and subsequent pasteurization processes on amino acids composition of orange juice[J].Plant Foods for Human Nutrition (Dordrecht,Netherlands),2015,70(2):153-159.
- [31]Liu Xiaofeng,Wang Shuo,Tamogami S,et al.Volatile profile and flavor characteristics of ten edible oils[J].Analytical Letters,2021,54(9):1423-1438.
- [32]Williamson J G,Lyrene P M.Blueberry varieties for Florida[D].Gainesville:University of Florida IFAS Extension,2004.
- [33]Motoki O,Chihiro H,Eisuke T,et al.Development for the simultaneous analytical method of furan and alkyl furans in processed foods[J].Journal of the Food Hygienic Society of Japan,2023,64(1):29-33.
- [34]Fan Xuetong.Impact of ionizing radiation and thermal treatments on furan levels in fruit juice[J].Journal of Food Science,2005,70(7):409-414.
- [35]Zhang Wentao,Li Xuejie,Wang Xuzeng,et al.Decoding the effects of high hydrostatic pressure and high-temperature short-time sterilization on the volatile aroma profile of red raspberry juice[J].Foods (Basel,Switzerland),2024,13(10):1574.
- [36]谭志伟,余爱农.反应温度和反应时间对抗坏血酸-赖氨酸Maillard反应体系中挥发性化合物形成的影响[J].食品科学,2012,33(16):124-129.
- [37]Li Ping,Callery P S,Gan Liangshang,et al.Esterase inhibition attribute of grapefruit juice leading to a new drug interaction[J].Drug Metabolism and Disposition:the Biological Fate of Chemicals,2007,35(7):1023-1031.
- [38]Guo Jingtong,Wu Lihong,Sun Yujing,et al.Power ultrasound enhanced the flavor quality of tomato juice[J].Journal of the Science of Food and Agriculture,2025,105(7):3722-3729.
- [39]Gaomi K,Yamasaki Y,Yamamoto H,et al.Characterization of a hydroperoxide lyase gene and effect of C6-volatiles on expression of genes of the oxylipin metabolism in Citrus[J].Journal of Plant Physiology,2003,160(10):1219-1231.
- [40]Perotti V E,Moreno A S,Trípodi K E J,et al.Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure[J].Physiologia Plantarum,2015,153(3):337-354.
- [41]Hu Gaofei,Zhu Yan,Hernandez M,et al.An efficient method for the simultaneous determination of furan,2-methylfuran and 2-pentylfuran in fruit juices by headspace solid phase microextraction and gas chromatography-flame ionisation detector[J].Food Chemistry,2016,192:914.