赵倩,丁志雯,钱亮亮,李甜,黄志发,房耀维,刘姝

• 1:江苏海洋大学食品科学与工程学院
• 2:连云港市质量技术综合检验检测中心

摘要(Abstract)：

关于海洋中产几丁质酶具有低温耐酸特性的研究报道极少，因此筛选出具有产低温耐酸特性的几丁质酶菌株具有现实意义。该研究从连云港连岛海域采集的海泥样品中，通过透明圈法筛选一株产几丁质酶的菌株CZW003,结合形态学特征观察、生理生化试验及16S rDNA扩增分析鉴定出菌株为还原亚硝酸盐寡养单胞菌(Stenotrophomonas nitritireducens)并作酶学特性研究。结果表明，该酶最适反应温度为35℃,且在5℃具有催化活性，在5～35℃之间酶活较为稳定；最适pH为6.0,在pH 5.0时酶活仍保持在95%以上，酸性环境下具有较好的稳定性；Mg~(2+)、Ca~(2+)、K~+对酶活具有促进作用，而Hg~+、Fe~(2+)、Ag~+及EDTA对酶活具有显著的抑制作用；用胶体几丁质为底物时酶活力最高。

关键词(KeyWords)： 几丁质酶;海洋细菌;Stenotrophomonas nitritireducens;低温;耐酸;酶学特性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31772016);; 江苏省高等学校自然科学研究重大项目(20KJA550001);; 江苏省“333高层次人才培养工程”;; 江苏省海洋资源与环境重点实验室开放课题(SH20191204)

作者(Author): 赵倩,丁志雯,钱亮亮,李甜,黄志发,房耀维,刘姝

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