响应面法优化超声辅助提取大果沙枣叶多酚工艺及其抗氧化和抗糖化能力研究Optimization of Ultrasonic-Assisted Extraction Process of Polyphenols from Leaves of Elaeagnus moorcroftii Wall. by Response Surface Methodology and Study on Their Antioxidant and Anti-Glycation Capacity
孙妍,高尚,郭玉婷,陈璐,薛雪,唐辉,廖兵武,贾瑞博
摘要(Abstract):
在推动循环经济的进程中,该研究着重于大果沙枣叶多酚的提取及其应用潜力。利用超声波辅助提取技术对大果沙枣叶中的多酚类化合物进行了提取工艺的优化,并对其抗氧化和抗糖化能力进行了全面评估。试验确定了最佳提取条件为超声时间20.00 min、料液比1∶53.40 (g/mL)、超声温度69.70℃、超声功率400 W,此条件下得到多酚提取量为(28.24±0.46) mg/g。抗氧化试验表明,该多酚对DPPH·、·OH和ABTS~+·具有较强清除能力,其IC_(50)值分别为0.112, 0.110, 0.146 mg/mL。在BSA-GLC模拟反应体系下,其糖化抑制率显著提高至(44.64±7.20)%。研究结果表明,超声辅助提取的大果沙枣叶多酚具有显著的体外抗氧化和抗糖化能力,其能力随着大果沙枣叶多酚浓度的增加而增强,表明其可作为潜在的天然食品添加剂,为沙枣副产品的综合利用提供了新方向。
关键词(KeyWords): 大果沙枣叶;多酚;超声辅助提取;响应面;抗氧化;美拉德反应
基金项目(Foundation): 广东省自然科学基金面上项目(2024A1515010576);; 新疆植物药资源利用教育部重点实验室主任基金(2021QDJF002)
作者(Author): 孙妍,高尚,郭玉婷,陈璐,薛雪,唐辉,廖兵武,贾瑞博
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