嗜盐四联球菌与多变假丝酵母强化发酵对蚕豆酱风味品质的影响Effect of Enhanced Fermentation by Tetragenococcus halophilus and Candida versatilis on Flavor Quality of Broad Bean Paste
汪坤,卢云浩,程杰
摘要(Abstract):
为探究郫县豆瓣内源性优势微生物嗜盐四联球菌(Tetragenococcus halophilus MK063724)与多变假丝酵母(Candida versatilis MK063708)强化发酵对蚕豆酱风味品质的影响,采用顶空固相微萃取-气相色谱-质谱联用技术(HS-SPME-GC-MS)分析不同接种方式强化发酵蚕豆酱的挥发性风味化合物差异。结果表明,所有样品中共检测出69种挥发性化合物,且不同发酵组挥发性化合物含量差异明显。相较于传统发酵组,嗜盐四联球菌和多变假丝酵母共接组、多变假丝酵母组和嗜盐四联球菌组的含量分别提升了60.49%、30.09%和27.05%,主要集中在酯类和醇类化合物含量的提升。偏最小二乘判别分析表明,同时接种嗜盐四联球菌和多变假丝酵母样品的香气属性显著区分于其他发酵组,结合气味活性值分析,同时接种两种菌可形成更丰富的特征香气物质,尤其是2-甲基丁酸乙酯、己酸乙酯、苯乙酸乙酯等酯类化合物,从而有利于花果香气的提升。该研究结果可为郫县豆瓣风味品质的提升提供理论指导。
关键词(KeyWords): 蚕豆酱;嗜盐四联球菌;多变假丝酵母;强化发酵;特征风味化合物
基金项目(Foundation): 四川省自然科学基金青年科学基金项目(2022NSFSC1624)
作者(Author): 汪坤,卢云浩,程杰
参考文献(References):
- [1]李幼筠.“郫县豆瓣”剖析[J].中国酿造,2008(11):83-86.
- [2]谭馨怡,卢云浩,任尧,等.不同陈酿时间下郫县豆瓣挥发性风味化合物及标志性风味成分解析[J].中国调味品,2021,46(10):43-46.
- [3]张芃芃,王世乐,陈福生.蚕豆豆瓣酱发酵过程中微生物群落与挥发性风味物质的相关性[J].中国酿造,2023,42(5):41-49.
- [4]卢云浩,何强.郫县豆瓣特征挥发性物质演变及其香型特性研究[J].中国食品学报,2021,21(4):326-335.
- [5]徐培,范文教,孙俊秀,等.甜瓣子发酵品质的研究现状[J].中国调味品,2022,47(10):206-209,220.
- [6]李恒,王泽亮,邓维琴,等.甜瓣子中生物胺产生菌株和降解菌株的筛选[J].中国食品学报,2022,22(10):190-198.
- [7]郑鹏飞,张丽杰,王栋,等.一种自下而上的合成微生物组理性构建策略,用于郫县豆瓣发酵剂设计[J].微生物学报,2022,62(10):3913-3931.
- [8]胡廷,范智义,张其圣,等.酵母菌强化发酵郫县豆瓣甜瓣子的研究[J].中国调味品,2020,45(7):76-80,85.
- [9]LI X Z,XU X Y,WU C Z,et al.Effect of sequential inoculation of Tetragenococcus halophilus and Wickerhamomyces anomalus on the flavour formation of early-stage moromi fermented at a lower temperature[J].Foods,2023,12(18):3509.
- [10]ZHANG L,HUANG J,ZHOU R Q,et al.The effects of different coculture patterns with salt-tolerant yeast strains on the microbial community and metabolites of soy sauce moromi[J].Food Research International,2021,150:110747.
- [11]索化夷,赵欣,骞宇,等.永川豆豉发酵过程中总糖和氨基酸变化与滋味的形成[J].食品科学,2015,36(21):100-104.
- [12]JIANG L,LU Y H,MA Y,et al.Comprehensive investigation on volatile and non-volatile metabolites in low-salt doubanjiang with different fermentation methods[J].Food Chemistry,2023,413(7):135588.
- [13]LU Y H,TAN X Y,LYU Y P,et al.Physicochemical properties and microbial community dynamics during Chinese horse bean-chili-paste fermentation,revealed by culture-dependent and culture-independent approaches[J].Food Microbiology,2020,85:103309.
- [14]CUI R Y,ZHENG J,WU C D,et al.Effect of different halophilic microbial fermentation patterns on the volatile compound profiles and sensory properties of soy sauce moromi[J].European Food Research and Technology,2014,239(2):321-331.
- [15]陈丽兰,陈祖明,袁灿.气相色谱-离子迁移谱结合化学计量法分析不同炒制时间对郫县豆瓣酱挥发性化合物的影响[J].食品科学,2023,44(14):283-290.
- [16]YANG M L,HUANG J,ZHOU R Q,et al.Characterization of the flavor in traditional Pixian Doubanjiang by polyphasic quantitative detection technology[J].Food Research International,2020,138:109753.
- [17]贾云.蚕豆酱发酵过程微生物群落结构及其功能分析[D].无锡:江南大学,2021.
- [18]刘平,王雪梅,向琴,等.郫县豆瓣智能后发酵工艺优化及品质分析[J].食品科学,2020,41(22):166-176.
- [19]陈丽兰,陈祖明,袁灿.郫县豆瓣炒制后挥发性风味物质的分析[J].中国调味品,2020,45(4):177-180.
- [20]LIN H B,LIU Y,HE Q,et al.Characterization of odor components of Pixian Douban (broad bean paste) by aroma extract dilute analysis and odor activity values[J].International Journal of Food Properties,2019,22(1):1223-1234.
- [21]冯云子.高盐稀态酱油关键香气物质的变化规律及形成机理的研究[D].广州:华南理工大学,2016.
- [22]张蕾蕾,吴剑荣,张洪涛,等.基于GC-MS结合化学计量学方法鉴定四川郫县豆瓣酱[J].食品与发酵工业,2022,48(13):268-276.
- [23]LU Y H,CHI Y L,LYU Y P,et al.Evolution of the volatile flavor compounds of Chinese horse bean-chili-paste[J].LWT-Food Science and Technology,2019,102(2):131-135.
- [24]GIRI A,OSAKO K,OKAMOTO A,et al.Olfactometric characterization of aroma active compounds in fermented fish paste in comparison with fish sauce,fermented soy paste and sauce products[J].Food Research International,2010,43(4):1027-1040.
- [25]DING W W,YE X Q,ZHAO X Y,et al.Fermentation characteristics of Pixian broad bean paste in closed system of gradient steady-state temperature field[J].Food Chemistry,2022,374(4):131560.
- [26]ZHU M,SUN J,ZHAO H A,et al.Volatile compounds of five types of unifloral honey in Northwest China:correlation with aroma and floral origin based on HS-SPME/GC-MS combined with chemometrics[J].Food Chemistry,2022,384(1):132461.
- [27]LI C,AI-DALALI S,WANG Z P,et al.Investigation of volatile flavor compounds and characterization of aroma-active compounds of water-boiled salted duck using GC-MS-O,GC-IMS,and E-nose[J].Food Chemistry,2022,386(1):132728.
- [28]ZHAO C,FAN W L,XU Y.Characterization of key aroma compounds in Pixian broad bean paste through the molecular sensory science technique[J].LWT-Food Science and Technology,2021,148(6):111743.