刘欣宁,卢云浩,何强

• 1:四川大学轻工科学与工程学院
• 2:成都大学食品与生物工程学院

摘要(Abstract)：

为揭示郫县豆瓣内源性优势菌株Bacillus amyloliquefaciens与Candida versatilis的互作机制，文章基于非靶向代谢组学和转录组学技术，探究了两株菌在单独培养和共培养体系下代谢物和基因表达水平的差异。结果表明，与单独培养B.amyloliquefaciens与C.versatilis相比，共培养体系下分别有193种和297种显著差异代谢物，集中在脂类和类脂分子、有机酸及其衍生物、氨基酸及其衍生物等。通过转录组学和差异代谢通路分析，B.amyloliquefaciens在共培养体系下上调表达基因801个，集中在生物过程中；而下调表达基因805个，以分子功能为主。C.versatilis对B.amyloliquefaciens的生长代谢具有双向作用，其中糖酵解/糖异生、脂肪酸降解等途径显著增强，而脂肪酸合成、氨基酸相关代谢显著减弱。研究结果可揭示两株菌的互作机制，并为改善郫县豆瓣风味及品质提供理论指导。

关键词(KeyWords)： 代谢组学;转录组学;Bacillus amyloliquefaciens;Candida versatilis;互作机制

Abstract:

Keywords:

基金项目(Foundation): 18H0481中国好粮油产学研合作协议(0030805501E24)

作者(Author): 刘欣宁,卢云浩,何强

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