顶空固相微萃取-气相色谱-质谱法测定大曲中的挥发性组分Determination of Volatile Constituents in Daqu Using Headspace Solid-phase Microextraction and Gas Chromatography-Mass Spectrometry
陈勇,陈泽军,周瑞平,黄钧,赵金松,秦臻,周荣清
摘要(Abstract)：
采用顶空固相微萃取-气质联用(HS-SPME/GC-MS)技术剖析大曲挥发性组分的特点,以有效峰数量和峰面积作为比较指标,考察了萃取头、萃取温度、萃取时间、样品用量以及萃取方式对萃取过程的影响,以期提高该技术对大曲挥发性组分的萃取效率。结果表明:采用85μm CAR/PDMS萃取头,大曲用量2.0g,萃取温度60℃,萃取时间50min以及不加溶剂的萃取方式对大曲挥发性组分的萃取效果较好。运用该条件在泸州老窖的中高温大曲样品中共检出挥发性组分34种,包括烯烃类2种,吡嗪类10种,芳香族类10种,酯类12种,分别占总数的5.88%,29.41%,29.41%,35.29%。
关键词(KeyWords)： 大曲;挥发性组分;顶空固相微萃取
基金项目(Foundation): 国家自然科学基金资助(31171742);; 四川省重大攻关项目资助(09ZC0735)
作者(Author): 陈勇,陈泽军,周瑞平,黄钧,赵金松,秦臻,周荣清
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