四川传统发酵香肠中几株优势乳酸菌的分离鉴定Isolation and Identification of Several Dominant Lactic Acid Bacteria in Sichuan Traditional Fermented Sausage
吉莉莉,陈雪玲,魏艳,付婷婷,何丹,张崟,赵黎明,王卫
摘要(Abstract):
为了获得产乳酸菌种,以制备用于制作聚乳酸的乳酸分子,以四川传统发酵香肠为原料,对其中的优势乳酸菌进行分离、纯化,并利用Biolog微生物鉴定系统的生理生化鉴定,结合16SrDNA分子生物学鉴定,对所得乳酸菌的种属进行了鉴定。结果显示,从发酵香肠中分离得到3株优势乳酸菌,经生理生化及分子生物学鉴定发现,所得3株乳酸菌分别为粪肠球菌(Enterococcus faecalis)、戊糖片球菌(Pediococcus pentosaceus)和肠膜明串珠菌(Leuconostoc mesenteroides)。该结果为制备生物源乳酸单体提供了菌种,并为开发肉制品发酵剂及传统肉制品加工提供了参考。
关键词(KeyWords): 四川传统发酵香肠;乳酸菌;分离;鉴定
基金项目(Foundation): 国家重点研发计划项目(2017YFB0309302)
作者(Author): 吉莉莉,陈雪玲,魏艳,付婷婷,何丹,张崟,赵黎明,王卫
参考文献(References):
- [1]王卫,宋浪,张佳敏,等.腊肠品质形成机理及其调控研究进展[J].肉类研究,2017,31(5):52-55.
- [2]褚福娟,孔保华,黄永.发酵肉制品常见乳酸菌的发酵性能研究[J].肉类研究,2009(6):16-20.
- [3]李玉珍,肖怀秋,林亲录.乳酸菌在发酵肉制品中的应用[J].肉类工业,2006(4):8-10.
- [4]冯刚,王华峰,张朝阁,等.聚乳酸的合成技术及其国内外应用分析[J].工程塑料应用,2016,44(11):131-135.
- [5]Rojan P,John K N,Ashok P.Fermentative production of lactic acid from biomass:an overview on process developments and future perspectives[J].Appl Microbiol Biotechnol,2007(3):524-534.
- [6]Frounchi M,Shamshiri S.Magnetic nanoparticles-loaded PLAPEG microspheres as drug carriers[J].Journal of Biomedical Materials Research:Part A,2015(5):1893-1898.
- [7]Pandey S K,Ghosh S,Maiti P,et al.Therapeutic efficacy and toxicity of tamoxifen loaded PLA nanoparticles for breast cancer[J].International Journal of Biological Macromolecules,2015(5):309-319.
- [8]张妮,李琦,侯振安,等.聚乳酸生物降解地膜对土壤温度及棉花产量的影响[J].农业资源与环境学报,2016,33(2):114-119.
- [9]王亭亭,李梅,江伟,等.聚乳酸地膜在大棚西瓜种植中的应用研究[J].农业资源与环境学报,2017(6):536-542.
- [10]张龙翼,晏宸然,张崟,等.聚乳酸的合成方法及其应用[J].农产品加工,2018(10):63-66.
- [11]白婷,吉莉莉,张佳敏,等.微生物发酵剂对冷藏四川腊肠特性的影响研究[J].食品科技,2018,43(11):149-155.
- [12]朱志远,卢士玲,孙晓斌,等.发酵剂对发酵香肠微生物和理化品质的影响[J].江苏农业学报,2009,25(4):890-893.
- [13]Giraffa G.Functionality of Enterococci in dairy products[J].International Journal of Food Microbiology,2003,88(2-3):215-222.
- [14]Cocconcelli P S,Cattivelli D,Gazzola S.Gene transfer of vancomycin and tetracycline resistances among Enterococcus faecalis during cheese and sausage fermentations[J].International Journal of Food Microbiology,2003,88(2-3):315-323.
- [15]Klibi N,Aouini R,Borgo F,et al.Antibiotic resistance and virulence of faecal Enterococciisolated from food-producing animals in Tunisia[J].Annals of Microbiology,2015,65(2):695-702.
- [16]Sparo M,Nuez G G,Castro M,et al.Characteristics of an environmental strain,Enterococcus faecalis CECT7121,and its effects as additive on craft dry-fermented sausages[J].Food Microbiology,2008,25(4):607-615.
- [17]张开屏,张保军,田建军.一株戊糖片球菌对发酵羊肉干品质的影响[J].食品研究与开发,2018,39(13):99-104.
- [18]黄金枝,杨荣玲,唐道邦,等.不同菌种组合对发酵广式香肠品质的影响[J].现代食品科技,2014,30(10):147-153.
- [19]Nie X H,Lin S L,Zhang Q L.Proteolytic characterisation in grass carp sausage inoculated with Lactobacillus plantarum and Pediococcus pentosaceus[J].Food Chemistry,2014,145:840-844.
- [20]Johansson G,Berdague J L,Larsson M.Lipolysis,proteolysis and formation of volatile components during ripening of fermented sausage with Pediococcus pentosaceus and Staphylococcus xylococcus as starter cultures[J].Meat Science,1994,38:203-218.
- [21]李俊霞,王卫,王新惠,等.发酵香肠风味物质的来源和形成[J].食品科技,2015,40(5):160-165.
- [22]李文斌,宋敏丽,高荣琨.肠膜明串珠菌的研究和应用进展[J].食品工程,2006(4):3-4,11.
- [23]庞志瑞.香肠的风味及颜色[J].天津食品科研,1981(2):10-15.