利用响应面方法优化光合细菌Rhodospirillum Rubrum S1培养基Optimization of Culture Medium for Rhodospirillum Rubrum S1 by Response Surface Methodology
潘艳,李师豪,周毓麟,王恩思,侯阿澧
摘要(Abstract)：
试验采用响应面方法优化光合细菌Rhodospirillum Rubrum S1培养基。结果表明:培养基成分中柠檬酸、牛肉膏、MgSO4和FeSO4对于光合细菌的生长影响最为显著。最优培养基配方:柠檬酸为3.5291g/L、牛肉膏为3.3190g/L、MgSO4为0.5083g/L、FeSO4为0.0194g/L。此时最大响应值为13.77mg/L。验证实验结果表明,采用优化后的培养基,光合细菌的类胡萝卜素产量达到13.69mg/L,与理论值相差0.58%。因此,利用响应面法对光合细菌的培养基进行优化合理可行。
关键词(KeyWords)： 光合细菌;响应面;类胡萝卜素
基金项目(Foundation):
作者(Author): 潘艳,李师豪,周毓麟,王恩思,侯阿澧
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