超声波辅助醇提法提取栀子中栀子黄色素Extraction of Gardenia Yellow Pigment from Gardenia by Ultrasonic-Assisted Ethanol Extraction Method
陈莎莎,曹如涛,林继辉
摘要(Abstract):
提高栀子果中栀子黄色素的提取率对后期栀子黄色素的精制及应用具有积极促进作用。该研究旨在建立一种从栀子果实中提取栀子黄色素的有效经济方法。通过超声辅助法研究栀子果实中粒度、提取时间、提取温度、提取剂浓度及液料比对栀子黄色素提取率的影响,基于单因素实验结果,采用五因素三水平的响应面法优化提取工艺,以提高栀子黄色素的提取率。结果显示,栀子黄色素的最佳提取工艺是提取时间31 min、提取温度62℃、液料比25∶1、乙醇浓度48%、粒度80目。采用响应面法优化后的提取工艺,栀子黄色素实际平均提取率为18.36%,与响应预测值18.38%相差0.02%,误差为0.19%,误差较小,说明该工艺具有良好的可靠性。与单因素实验相比,优化后的提取工艺将栀子黄色素的提取率从15.15%提高到18.36%,故优化后的工艺有利于栀子黄色素的提取,具有实际应用价值。
关键词(KeyWords): 栀子果;栀子黄色素;超声波;响应面法;优化
基金项目(Foundation): 福建省教育厅中青年教师教育科研项目(JAT200972);; 泉州市高等职业教育技能名师工作室(QZGZS-2019-02)
作者(Author): 陈莎莎,曹如涛,林继辉
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